buffer lumiprobe catalog no 61150 Search Results


buffer  (Lumiprobe)
93
Lumiprobe buffer
Buffer, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+lumiprobe+catalog+no+61150/Click+chemistry+buffer/pmc12648639-402-96-97
Average 93 stars, based on 1 article reviews
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93/100 stars
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ascorbic acid  (Lumiprobe)
94
Lumiprobe ascorbic acid
Ascorbic Acid, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cy3b azide  (Lumiprobe)
93
Lumiprobe cy3b azide
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Cy3b Azide, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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azidobutyric acid nhs ester  (Lumiprobe)
94
Lumiprobe azidobutyric acid nhs ester
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Azidobutyric Acid Nhs Ester, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad cat  (Bio-Rad)
96
Bio-Rad bio rad cat
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flamingo fluorescent protein gel stain  (Bio-Rad)
94
Bio-Rad flamingo fluorescent protein gel stain
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Flamingo Fluorescent Protein Gel Stain, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+lumiprobe+catalog+no+61150/Flamingo+Fluorescent+Protein+Gel+Stain/pmc12648639-402-135-140
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flamingo fluorescent protein gel stain - by Bioz Stars, 2026-09
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bio spin p  (Bio-Rad)
96
Bio-Rad bio spin p
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Bio Spin P, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+lumiprobe+catalog+no+61150/Micro+Bio-Spin+P-6+Gel+Columns/pm37038000-208-308-313
Average 96 stars, based on 1 article reviews
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acrylamide solution  (Bio-Rad)
97
Bio-Rad acrylamide solution
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Acrylamide Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ssc buffer  (Bio-Rad)
94
Bio-Rad ssc buffer
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Ssc Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad catalog no 161 0140  (Bio-Rad)
99
Bio-Rad bio rad catalog no 161 0140
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Bio Rad Catalog No 161 0140, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+lumiprobe+catalog+no+61150/40%25+Acrylamide+Solution/pm37038000-208-230-230
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bio rad catalog no 161 0140 - by Bioz Stars, 2026-09
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glass bottom 24 well plates  (Greiner Bio)
94
Greiner Bio glass bottom 24 well plates
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Glass Bottom 24 Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fbs  (Danaher Inc)
96
Danaher Inc fbs
a–f SpeedyTrack data of <t>Cy3B-labeled</t> carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.
Fbs, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a–f SpeedyTrack data of Cy3B-labeled carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.

Journal: Nature Communications

Article Title: Direct microsecond wide-field single-molecule tracking and super-resolution mapping via CCD vertical shift

doi: 10.1038/s41467-025-65529-x

Figure Lengend Snippet: a–f SpeedyTrack data of Cy3B-labeled carbonic anhydrase diffusing in PBS, with an exposure time of 300 µs and a vertical shift time of 7.5 µs for 15 rows at each timepoint. a A portion of one CCD frame, showing multiple streaks from single molecules entering/exiting the focal plane at different locations and timepoints. b Zoom-in of one streak, with colored boxes indicating different time points. c Single-molecule trajectory reconstructed from the localizations in ( b ) by back-shifting 15 rows between consecutive timepoints, colored as the boxes in ( b ). d Additional example trajectories. e Distribution of durations for single-molecule trajectories collected over 4000 SpeedyTrack frames. f Squares: MSD at different time lags calculated from the trajectories. Error bars: standard errors. Line: Linear fit to the first 4 data points, yielding a diffusion coefficient of 90 ± 3 µm 2 /s (95% confidence interval) from 5573 tracks. Inset : distribution of apparent diffusion coefficients estimated for individual trajectories longer than 12 timesteps. g–j SpeedyTrack data of Cy3B free dye. g A portion of one SpeedyTrack frame showing two single-molecule streaks for Cy3B diffusing in PBS, with exposure and vertical-shift times of 50 and 7.5 µs at each timepoint. h Examples of reconstructed Cy3B trajectories in PBS and acetone at 57.5 and 50 µs timesteps, respectively. The arrow points to the trajectory reconstructed from the long streak in ( g ). i Data points: MSD vs. time lag for Cy3B in PBS (black, 1860 tracks) and acetone (red, 1924 tracks). Error bars: standard errors. Lines: Linear fits to the first 4 data points, yielding diffusion coefficients of 378 ± 9 and 1022 ± 30;µm 2 /s (95% confidence intervals), respectively. j Circles: SpeedyTrack-measured diffusion coefficients of Cy3B in solvents and mixtures of varying viscosities. Each data point is obtained from the MSD-time lag fit to one experiment of 10 3 − 10 4 tracks (Supplemental Table ). Error bars: 95% confidence intervals. Dashed curve: Trend predicted by scaling the PBS value by the medium viscosity. Inset : Results in methanol-water mixtures of varying methanol mole fractions, compared to the expected trend based on the known mixture viscosity.

Article Snippet: 17 μM of this DNA hairpin was mixed with 50 μM Cy3B azide (Lumiprobe 19330), 0.5 mM tris(benzyltriazolylmethyl)amine (TBTA), and 0.5 mM ascorbic acid in 30 μL copper(II)-TВTA catalytic buffer (Lumiprobe 61150) and allowed to react overnight.

Techniques: Labeling, Diffusion-based Assay, Viscosity